O que as personaxes len: Alusións literarias na pequena pantalla

Literature is present in TV fiction through adaptations, but literary works are also featured directly onscreen, sometimes serving as a homage showrunners pay to their literary sources, sometimes to set the mood of a particular episode or a season arc, sometimes for a didactic purpose. This article intends to examine in depth the symbiotic relation between both media, using the analysis of some examples to do so, and thus prove that intermediality not only contributes to elevate the TV series but it also provides the literary works the series refers to with new life.A literatura está presente na ficción televisiva a través das adaptacións, pero as obras literarias tamén se amosan de forma directa na pantalla, ben como homenaxe ás fontes das que beben os creadores, para establecer a atmósfera dun episodio ou dun arco argumental, ou cun fin didáctico. Mediante a análise dalgúns exemplos, este artigo tentará profundar na relación simbiótica entre ambos medios e demostrar que esta intermedialidade non só é enriquecedora para a serie televisiva, senón que tamén dota de nova vida as obras literarias que esta amosa

Synthesis and electrochemical study of new 3-(hydroxyphenyl)benzo[f]coumarins

New hydroxyl substituted 3-arylbenzo[f]coumarins (compounds 6–10) have been designed and synthesized. Their electrochemical redox mechanisms, and the influence of one or two hydroxyl groups, in different positions on the coumarin scaffold, was investigated by cyclic, differential pulse and square wave voltammetry, at a glassy carbon electrode, at different pHs, and a comparative study was performed. The structural information obtained enabled a better understanding of the structure/electrochemical relationship of hydroxyl substituted 3-arylbenzo[f]coumarins, compounds with important antioxidant properties

Imigração e arte: o acúmulo de documentos e objetos como forma de construção de si

Nas décadas de 1930 e 1940 o Brasil recebeu uma leva de migrantes alemães judeus fugindo da ascensão do nacional socialismo ao poder. A entrada desses migrantes de guerra mudou a paisagem intelectual brasileira. A chegada a um novo mundo é também o encontro com o vazio de uma identidade a ser redescoberta. Nesse trabalho investigo o processo de constituição de um acervo de documentos e objetos como forma de construção de uma identidade migrante. A coleção de Olly e Werner Reinheimer, alemães migrados para o Brasil na década de 1930, é o campo empírico para observação das negociações identitárias entre a memória inscrita nos documentos acumulados e nas pessoas entrevistadas e as memórias coletivas que se cristalizaram em livros, documentários e arquivos públicos

Insights into the antioxidant activity of phenolic compounds: Synthesis and electrochemical study of new series of hydroxycoumarins

The 15th International Electronic Conference on Synthetic Organic Chemistry session Bioorganic, Medicinal and Natural ProductsPhenolic compounds are bioactive substances widely distributed in the vegetable kingdom. They act as natural antioxidants, and their presence contributes to the color, flavor and aroma of food. This group of micronutrients is composed of one or more aromatic benzene rings with one or more hydroxyl groups and their redox properties are related with their chemical structure characteristics. The knowledge of their redox potentials may help the food industry, because when phenolic compounds are oxidized they could affect the quality of the wines, beers, grape juices, etc. Coumarins are a large family of compounds, of natural and synthetic origin, that show important biological activities. Therefore, they occupy an important place in the study of natural products and synthetic organic chemistry. Recent studies pay special attention to their antioxidative, anticarcinogenic and enzymatic inhibition properties. Their preparation, and the versatility of the synthetic methodology, allowed us obtaining a wide family of compounds with substituent in different positions in the molecule. The election of these derivatives has considered the later pharmacological evaluation. The investigation of the properties of these compounds, the study of the structural pattern and the elucidation of their biological role is of great interest for further development of coumarin-like antioxidant drugs. The electrochemical behaviour of a group of differently substituted hydroxycoumarins was investigated using cyclic, differential pulse and square wave voltammetry, in aqueous media at a glassy carbon electrode over the whole pH range. The antioxidant reactivity and capacity were also evaluated through a competition assay with hydroxyl radical (OH•) and DMPO like ORAC-FL methodology. Number and positions of the hydroxyl groups were important factors in the antioxidant activities against peroxyl radical of both types of coumarins derivativesWe are grateful to the Xunta de Galicia (PGIDIT09CSA030203PR) and Ministerio de Sanidad y Consumo (FIS PS09/00501) for the partial financial support. M.J.M. and A.G. also thank Fundação de Ciência e Tecnologia for the fellowship

Synthesis and pharmacological evaluation of coumarins as new scaffold on the Parkinson´s disease

The 13th International Electronic Conference on Synthetic Organic Chemistry session Bioorganic Chemistry and Natural ProductsWith the aim to find out the structural features for the MAO inhibitory activity and selectivity, in the present communication we report the design, synthesis and pharmacological evaluation of a new series of 8-bromo-6-methyl-3-phenylcoumarin derivatives without substituent and with different number of methoxy substituent in the 3-phenyl ring. The substituent in this new scaffold was introduced in the 3', 4' and/or 5' positions of the 3-phenyl ring of the coumarin moiety. The synthesized compounds 3-6 were evaluated as MAO A and B inhibitors using R-(-)-deprenyl (selegiline) and Iproniazide as reference inhibitors, showing, most of them, MAO-B inhibitory activities in the nanomolar range. Compounds 3 (11.05±0.81 nM), 4 (3.23±0.49 nM) and 5 (7.12±0.01 nM) show higher activity than selegiline (IC50 = 19.60 nM), and high MAO-B selectivity with 9,050- fold, 30,960-fold and 14,045-fold inhibition levels, with respect to the MAO-A isoformWe are grateful to the Spanish Ministerio de Sanidad y Consumo (PI061457 and PI061537) and to Xunta da Galicia (BTF20303PR, PXIB203022PR and CSA019203PR) and Fondazione Banco Sardegna (Italy) for financial support. M.J.M. also thanks MIUR for a PhD gran

Interference of bioassay methods on the results of entomopathogenic fungi selection for insect control

O objetivo deste trabalho foi avaliar a interferência dos métodos de bioensaios nos resultados de seleção de fungos entomopatogênicos, com a utilização de quatro isolados de Beauveria bassiana e adultos de Alphitobius diaperinus. Quanto à forma de inoculação, foram testados os seguintes métodos: imersão do inseto em suspensão de conídios; pulverização da suspensão sobre insetos; e tratamento de superfície com a suspensão em oito concentrações. Para avaliar a interferência do tempo de imersão sobre a mortalidade, os insetos foram colocados em suspensão de conídios por 1, 10, 20, 30, 40, 50 e 60 segundos. Conídios produzidos em arroz, insetos e meio sintético foram utilizados para avaliar a influência do substrato na virulência dos isolados. Os isolados UNIOESTE 4 e CG 152 foram os mais virulentos pelos métodos imersão e pulverização, respectivamente; em tratamento de superfície, a mortalidade foi baixa para todos os isolados. Observou-se que a mortalidade dos insetos é proporcional ao tempo em que ficam imersos, com tendência a se estabilizar após 40 segundos. Os isolados CG 71 e CG 152, produzidos sobre insetos, foram menos virulentos. Os métodos utilizados podem influenciar os resultados de bioensaios de seleção de fungos para o controle de insetos.The objective of this work was to evaluate the interference of bioassay methods on the entomopathogenic fungi selection, using four Beauveria bassiana isolates and Alphitobius diaperinus adults. Regarding the inoculation form on insect mortality, the following methods were tested: insects immersion in a conidia suspension; spraying of a suspension over the insects; and surface treatment with eight concentrations. In order to evaluate the interference of immersion time on mortality, insects were immersed in a fungal suspension for 1, 10, 20, 30, 40, 50, and 60 seconds. Conidia produced on rice, insects, and synthetic medium were used to evaluate the influence of substrate on the virulence of the isolates. UNIOESTE 4 isolate and CG 152 were the most virulents for the methods immersion and spray, respectively; however, mortality was low in all isolates for the surface treatment. Mortality was proportional to the time during which insects stayed immersed, with a tendency to become stable after 40 seconds. Conidia from isolates CG 71 and CG 152 produced on insects were less virulent. The methods may influence bioassay results in the fungi selection for insects control

Design, Synthesis and Pharmacological Evaluation of New Coumarin Derivatives as Monoamine Oxidase A and B Inhibitors

The 12th International Electronic Conference on Synthetic Organic Chemistry session Bioorganic Chemistry and Natural ProductsWith the aim to find out the structural features for the MAO inhibitory activity and selectivity, in the present communication we report the design, synthesis and pharmacological evaluation of a new series of coumarin derivatives with 4-methyl or cycloalkene or benzene ring condensed in the 3,4 position. The substituents in this new scaffold were introduced in the 5, 7 and/or 8 positions of the coumarin moiety. The synthesized compounds 1-13 were evaluated as MAO A and B inhibitors using clorgyline and selegiline, respectively, as reference inhibitors, showing, most of them, activities in the nanomolar range. Compounds 6 (IC50 = 1.18 nM) and 10 (IC50 = 1.48 nM), show higher activity than selegiline (IC50 = 19.60 nM), and high MAO-B selectivity with 100-fold and 1600-fold inhibition levels, with respect to the MAO-A isoformWe are grateful to the Xunta de Galicia (INCITE07PXI203030ES, PGIDIT05BTF20302PR and INCITE08PXIB203022PR) and Ministerio de Sanidad y Consumo (FIS PI061537 and PI061457) for financial support

Clinicopathological features and prognostic significance of CTNNB1 mutation in low-grade, early-stage endometrial endometrioid carcinoma

Low-grade and early-stage endometrioid endometrial carcinomas (EECs) have an overall good prognosis but biomarkers identifying patients at risk of relapse are still lacking. Recently, CTNNB1 exon 3 mutation has been identified as a potential risk factor of recurrence in these patients. We evaluate the prognostic value of CTNNB1 mutation in a single-centre cohort of 218 low-grade, early-stage EECs, and the correlation with beta-catenin and LEF1 immunohistochemistry as candidate surrogate markers. CTNNB1 exon 3 hotspot mutations were evaluated by Sanger sequencing. Immunohistochemical staining of mismatch repair proteins (MLH1, PMS2, MSH2, and MSH6), p53, beta-catenin, and LEF1 was performed in representative tissue microarrays. Tumours were also reviewed for mucinous and squamous differentiation, and MELF pattern. Nineteen (8.7%) tumours harboured a mutation in CTNNB1 exon 3. Nuclear beta-catenin and LEF1 were significantly associated with CTNNB1 mutation, showing nuclear beta-catenin a better specificity and positive predictive value for CTNNB1 mutation. Tumours with CTNNB1 exon 3 mutation were associated with reduced disease-free survival (p = 0.010), but no impact on overall survival was found (p = 0.807). The risk of relapse in tumours with CTNNB1 exon 3 mutation was independent of FIGO stage, tumour grade, mismatch repair protein expression, or the presence of lymphovascular space invasion. CTNNB1 exon 3 mutation has a negative impact on disease-free survival in low-grade, early-stage EECs. Nuclear beta-catenin shows a higher positive predictive value than LEF1 for CTNNB1 exon 3 mutation in these tumoursThis research was funded by the Instituto de Salud Carlos III (ISCIII) (PI17/01723), co-financed by the European Development Regional Fund ‘A way to achieve Europe’ (FEDER

Síndrome de Leigh e implicações para prática fisioterapêutica: relato de caso

Introdução: A Síndrome de Leigh (SL) é considerada uma doença neurodegenerativa ocasionada por alteração enzimática, que afeta diretamente os sistemas nervoso, muscular e respiratório. Objetivo: Avaliar e fornecer subsídios aos fisioterapeutas em relação à função pulmonar, força muscular respiratória e postura em uma adolescente portadora de SL. Relato do Caso: Foi realizado relato de caso de uma adolescente do sexo feminino, 15 anos de idade, portadora de SL, onde foram feitos testes de espirometria, manovacuometria e fotogrametria. A adolescente com SL apresentou fraqueza muscular respiratória, distúrbio respiratório restritivo moderado e alterações posturais, como a anteriorização da cabeça, assimetria em relação à tomada de peso e centro de gravidade. Conclusão: É necessário o acompanhamento fisioterapêutico ao longo da vida dessas crianças, tratando não somente a função motora, mas também respiratória. Os resultados colaboram com conhecimento sobre o tema e, assim, com a prática clínica, porém mais estudos são necessários

Associated control of Alphitobius diaperinus and effect of effective microorganisms in the development of Beauveria bassiana

O objetivo deste trabalho foi avaliar a eficiência de Beauveria bassiana (Bb), terra diatomácea (TD) e microrganismos eficazes (EM-4), associados ou não, no controle de Alphitobius diaperinus, e o efeito de EM-4 no desenvolvimento de B. bassiana. Os agentes de controle (Bb, TD e EM-4), em diferentes concentrações e combinações, foram aplicados em uma mistura de cama-de-frango e ração, em que os insetos foram acondicionados por dez dias. Avaliaram-se, in vitro: a germinação, as unidades formadoras de colônia (UFC), o crescimento vegetativo e a produtividade de conídios de Bb em contato com suspensão aquosa de EM-4 (1%) não filtrada e filtrada. Os maiores índices de mortalidade foram observados nos tratamentos TD + Bb + EM-4 e TD + Bb, nas maiores concentrações. Verificou-se efeito não-aditivo sinérgico para TD + Bb nas três concentrações. Nos testes com EM-4 filtrado, não houve diferença em relação à testemunha quanto à germinação e às UFC, entretanto, o crescimento vegetativo e a produtividade de conídios foram negativamente afetados. O uso conjunto de B. bassiana e terra diatomácea, para o manejo de populações de A. diaperinus, pode reduzir o uso de produtos químicos.The objective of this work was to evaluate Beauveria bassiana (Bb), diatomaceous earth (TD) and effective microorganisms (EM-4), associated or not, to Alphitobius diaperinus control, and the EM-4 effect on B. bassiana development. The control agents (Bb, TD and EM-4), in different concentrations and combinations, were applied in a mixture of litter and chicken food, where insects had been left per ten days. Conidia germination, colony-forming units (CFU), vegetative growth and the productivity of Bb conidia were evaluated when in contact with a filtered and non filtered watery EM-4 suspension (1%). The highest indices of mortality were observed in the treatments TD + Bb + EM and TD + Bb in the highest concentrations. A synergic non additive effect was observed for TD + Bb in the three concentrations. In filtered EM-4 tests, no difference was observed related to the control of germination and CFU, however, vegetative growth and conidia productivity were negatively affected. The joint use of B. bassiana and diatomaceous earth for A. diaperinus populations management can minimize the use of chemical products